FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701806
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5800.047.1714978
GTCTTAC5050.038.5316621
GTCTTGC14100.036.8009261
CTGTCGC8500.035.5757839
GTCTCGC7700.033.694361
TCGCTCT7350.033.3054774
GTTTCAC20950.031.3005243
GGGTTTC23800.030.8864921
TTCACCG5450.027.742585
TTGCTCT19800.027.635834
GATCCGT1856.1418177E-627.2427166
TCTCGCT9550.027.1671752
GGTATAC2955.18412E-1026.8729931
CTCGCTC8900.026.7188783
GTGTTAC5750.026.3206621
GGTTTCA22200.025.9706232
GTATTAC3900.025.8707351
CTGTTAC5600.025.7387431
TATACTG5050.025.6628685
TAACACG3109.313226E-1025.5478924
Produced by FastQC (version 0.11.5)