FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701806
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6250.040.318338
GTCTCGC6500.039.8776131
GTCTTGC14250.037.8953931
GTACTAG2750.036.655181
GTCTTAC5500.036.655181
ATACCCG1301.7362358E-533.2293934
CTGTCGC8800.031.9077729
TCGCTCT7800.030.4602784
GTGTTAG10300.030.0587751
TTGCTCT18500.028.798814
GTATTAT3550.028.394861
CTCGCTC8900.028.3134353
TTACACT5900.028.0666334
TAGTACT2853.5106495E-1027.7883224
TCTCGCT9100.027.692792
GTACTAC2354.5993147E-827.5749451
GGTCCGT2105.302936E-727.4274356
GTATTAA4000.027.0004671
CTTGCTC17300.026.6347373
TCACTCT17100.026.5252154
Produced by FastQC (version 0.11.5)