FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766322
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6550.043.9681058
GTCTTGC15000.037.000751
GTTTCAC23700.035.580473
GGGTTTC25200.035.181611
GTCTCGC7300.034.5586131
CTGTCGC8600.033.487349
GTCTCAC18400.031.73061
GTACCGA1153.2728564E-431.3388651
GTGTAAC4900.030.8911691
GGTTTCA24900.030.6843992
GTAATAC4000.030.6311363
TAATACT4250.030.4931764
GTACTAG2851.0913936E-1130.349221
GTATTAA3850.029.9550721
GGTATAC3201.8189894E-1229.2822551
TCGCTCT8900.029.1226964
TACACTG8750.028.799115
TTGCTCT18450.028.4869274
TTCACCG5700.027.7886185
TCTCGCT8850.027.6915192
Produced by FastQC (version 0.11.5)