FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766322
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6600.038.180928
GTCTCGC7200.035.0021481
GGGTTTC24600.034.2460061
TCGCTCT7650.032.94044
GTGTTAG8750.032.0933951
GTCTTGC13900.031.599061
GTTTCAC22650.031.4694583
GTATTAG4850.031.1771721
GTATTCG1402.8796698E-530.8590371
GTACCGC1504.6113055E-528.8017651
CCGCTTA1000.006285334528.7993229
GTGTAAC5350.028.2634161
ACCGTGT5400.027.999348
TTCACCG4900.027.917715
GTTAGCC7000.027.7707733
GTGTTAA5550.027.2449151
GGTTTCA25700.026.8965912
GGTATAC2955.2386895E-1026.8491021
TTGCTCT18250.026.8267634
GTGTATA7950.026.265761
Produced by FastQC (version 0.11.5)