FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034814
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7250.038.7304538
GTCTCGC9300.037.189061
TCGCTCT8750.036.205174
GTCTTGC15800.034.658791
TATACTG5650.033.1322445
GTGTTAC7050.032.7052731
TCTCGCT10550.030.733852
CGCTCTG11150.029.7035885
GTATAAT4400.029.4765571
CTGTCGC10050.029.3726949
CTCGCTC11250.028.8179783
TAATACT4100.028.097144
TACCTCG2054.295398E-728.097145
GGGTTTC30200.027.6762961
GTGTAAT7850.027.5364881
GTCTTAC6850.027.3488881
GGTTAAT4950.026.2013841
GTATAAC3358.0035534E-1125.810321
TAACACT6750.025.5996154
GTTTCAC26750.025.5860583
Produced by FastQC (version 0.11.5)