FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034814
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6950.035.222798
GTCTTGC15550.033.802081
TCGCTCT6650.033.5636864
GCGTTAC1751.0729673E-732.9156841
GTCTCGC7000.031.887071
GTCTTAC7000.031.887071
ACCGTGT5400.030.6664628
GTATTAT5250.028.8012261
GGGTTTC29300.028.506331
GTGTTAG11250.028.1611981
GGTATAC4350.028.1391261
CTGTCGC9300.027.8707839
GTCTCGA17000.027.5292286
CTCGATC7850.027.515748
GTACTAT3950.027.3429341
CCGTGTT6350.027.2124189
GTATTAC4250.027.1070351
TATACTG5600.026.9998195
TTACTCT8850.026.8472794
GTACTCG1358.419432E-426.6664896
Produced by FastQC (version 0.11.5)