FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427746
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.043.366818
GTCTTGC28050.039.612641
GTCTCGC13050.039.2548871
TCGCTCT13950.035.6212274
GTACTAC4700.032.2379461
TCTCGCT14900.031.9228972
TTGCTCT37700.031.9013544
CTGTCGC17700.030.5084849
CGCTCTG16850.028.6290875
GTTTCAC41150.028.5442093
CTTGCTC37150.027.9321213
CTCGCTC17700.027.684453
GGGTTTC45750.026.9681361
GTGTTAC11950.026.5662751
TCGATCT13600.026.4705969
TCTCGAT13450.026.2304927
CTCGATC13450.026.2304928
GTCTCGA29600.026.0270376
TCTTGCT42050.025.5367072
GTTAGCC17250.025.0646933
Produced by FastQC (version 0.11.5)