FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3427746
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13000.041.7886121
TCTGTCG14300.039.268338
TCGCTCT15350.037.5213244
GTCTTGC28100.037.1189961
TACACCG2650.035.31685
TCTCGCT16850.032.0448272
CTCGCTC17350.030.7063923
CGCTCTG16950.030.1559165
GTTTCAC38450.029.7712693
CTGTCGC20800.029.76599
GTATTAC6750.028.9734381
TTGCTCT38150.028.1182524
GGGTTTC41550.028.066931
TACACTG16700.027.1586365
GTATTAA8550.026.2624741
GTATTAG9450.026.0607661
GTCTCAC35500.025.0967081
CTTGCTC36250.024.8255313
GTATTAT11800.024.5537621
GTGTTAG19850.024.448621
Produced by FastQC (version 0.11.5)