FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2299081
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.044.8636868
GTCTCGC9000.041.6292881
TCGCTCT10750.040.185484
GTCTTGC17950.035.32291
CTGTCGC12800.034.3120169
TACACCG2104.0745363E-1034.285235
GTACCGT1601.9829786E-631.4995546
GTTTCAC29050.030.7515093
TCTCGCT11950.030.7496662
GGGTTTC32400.030.4658781
CGCTCTG11850.029.1641455
GTGTAAA13400.027.9599691
CTCGCTC12700.027.796063
CTCGATC8550.026.9469858
GTATTAC4800.025.5179521
GTATAAC4250.025.4296531
ACCGTGT7650.025.4114028
TTGCTCT26100.025.3789524
TCGAACT13450.025.1594949
TCTCGAT8700.024.8272327
Produced by FastQC (version 0.11.5)