FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253680
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT1105.524469E-639.271836
TCTGTCG8550.038.735968
GTCTTGC17450.038.4150851
GTCTCGC10900.037.0318831
TCGCTCT11050.033.2300154
TAGTACT4550.031.647634
TTCACCG6550.029.678715
ACCGTGT6400.029.249338
GTTTCAC27150.028.9349463
TTATACT4000.028.7993434
GGGTTTC32100.028.7421461
CTGTCGC12100.028.5613339
AATGGCG13400.028.4769615
GTACTAG4350.028.1691651
TCTCGCT12550.027.5684152
CGCTCTG12200.027.1469235
TCTAATA4250.027.135972
TTACTCT9700.026.7210394
TCTGTCA23350.026.51768
TTTACGC1100.00995739526.2079683
Produced by FastQC (version 0.11.5)