FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253680
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.056.4189228
GTCTTGC18400.043.046631
GTCTCGC10050.040.838811
CTGTCGC12700.040.250459
TCGCTCT10950.038.1355484
GTGTTAG11250.032.642391
GTATTAG5550.032.4348071
GTTAGCC10300.031.4551263
TTGCTCT23400.031.3834324
TTCACCG6050.030.940985
CTCGCTC12400.030.7730313
GTTTCAC27450.030.4250813
TGTTAGC11400.029.6863842
CTTGCTC23650.029.5295433
CGCTCTG13500.029.3322265
GTATTAC4250.028.8021091
GTGTAAT8050.028.6232151
GGGTTTC31250.028.1108571
TCTCGCT13250.027.7152352
GTCTTAC7350.027.4305781
Produced by FastQC (version 0.11.5)