FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589647
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7350.052.8991628
GTCTTGC12450.041.6460531
CTGTCGC9350.041.583839
TTGCTCT17450.033.4209144
GTCTCGC7150.033.236751
GGGTTTC22600.031.226861
TCGCTCT7400.031.1348614
GTACCGT1204.2272688E-429.9997396
CTTGCTC17100.029.0523783
GTTTCAC20350.029.0120323
TCGCACT1753.9857805E-628.7997464
GTCTAAG4700.027.5794321
GTACTAG2654.9021764E-927.1747021
GTGTTAA5350.026.9207321
GTACCGC1358.409443E-426.6714671
TTCACCG4600.026.6084615
GGTCTAC3800.026.5310921
GTATATA5750.026.3003851
GGATAAT5550.025.9506171
TATACTT3358.185452E-1125.790825
Produced by FastQC (version 0.11.5)