FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014569
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT9050.046.1427844
TCTGTCG7650.045.175638
GTCTCGC10100.042.811861
CTCGCTC11500.036.9678083
TCTCGCT11350.036.8272
GTCTTGC15400.035.565351
CGCTCTG11300.033.1321265
GTACTAT3750.032.6702041
GTATTAG5550.032.4624441
CTGTCGC11100.032.4318289
GTTTCAC24000.030.0232873
ACCGTGT5950.029.0414758
GTACCGT1555.7695637E-527.870456
TCTGTCA19400.027.8345328
GGGTTTC26950.027.543091
ACCATGT21800.027.4123338
GTATTAT5300.027.1949541
TTGCTCT21800.027.0820644
TACCGTT1358.419986E-426.666177
GTATTAC4350.026.5072671
Produced by FastQC (version 0.11.5)