FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447634
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.053.615528
GTCTCGC9650.044.023991
CTGTCGC13500.036.7989739
GTATTAC4450.035.598151
TCGCTCT10650.033.801184
GTCTTGC19550.033.148241
TCGTACT1153.295378E-431.3028354
CTCGCTC11850.029.1631473
GGTATAC5950.029.044171
GTGTAAC7450.028.995441
CGCTCTG12400.028.4502375
TCTCGCT13250.028.25872
GTGTTAG12850.028.0176431
GTGTTAC9400.026.8105011
GTGTAAA13850.026.5146031
GTGTAAG10400.026.3096451
TATACTG6300.026.2844415
GTACTAG4150.026.0260281
GGGTTTC32150.025.9801541
TTACACT12950.025.574054
Produced by FastQC (version 0.11.5)