FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235057
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.044.895468
TGTACCG1752.6011548E-937.029685
CTGTCGC11600.032.8967979
TCTCGAT8650.032.4633947
GTCTTGC18500.030.8123531
GTCTCGC10100.030.7196711
GTGTTAC7900.030.140911
ACCGTGT6700.030.090458
TCGATCT8650.029.1331639
CTCGATC8900.029.1244648
TTACACT8750.028.8098834
TAATACT5450.027.7526424
GTGTTAG12900.026.8485621
GTATAAC3505.456968E-1226.800621
GTGTAAT8800.026.2383691
GTATTAG7000.025.7698251
TAATCCG1400.00104086325.7150525
GGGTTTC30400.025.6341951
GTTTCAC27300.025.606673
TAGTACT5100.025.4204864
Produced by FastQC (version 0.11.5)