FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123900
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.045.0407078
GTCTCGC11800.037.2892651
GTCTTGC25700.036.7683531
TCGCTCT13200.033.826024
GTACTAG5800.031.0919511
CTGTCGC17600.031.0911439
CTCGCTC14350.028.6178443
TCTCGCT12950.028.3763222
TTGCTCT34400.027.634314
GTATTAT9550.027.1916221
GTCTCAC30000.026.4488871
GTATTAC6700.025.8388041
CTTGCTC34600.024.9872863
GTATTAA9000.024.8459261
TTACACT13350.024.8147374
GTATAAT8500.024.6101951
CGCTCTG15550.024.5407715
GGGTTTC42800.024.1006221
GTTTCAC39650.023.9852733
GTATTAG9700.023.7965621
Produced by FastQC (version 0.11.5)