FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123854
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6200.027.0960458
CATATCG851.309351E-424.70638370
GTATTAA3050.022.9556061
CTGTCGC8200.022.6218769
GTATTAC2800.022.5046941
TCGCTCT7850.022.2981684
ATTATCG800.002668300421.87441355
GTCTCGC7900.021.7133881
TGTCGCC8550.021.69583310
GTCTTGC10900.021.5182481
GTATTAT3350.020.8998811
TCTCGCT8250.020.3678842
TACCCCG1558.7369335E-720.32735
CTCGCTC7750.020.326823
GTCGCCC7700.019.09039511
GTATAGG3001.8189894E-1218.670561
CGCACGT950.007202917218.425335
ATTACGT1150.001006990118.2646773
GTGTAAT4800.018.232971
GGTATAC2701.2369128E-1018.1519341
Produced by FastQC (version 0.11.5)