FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123854
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5350.028.1307568
GAGTACG657.9507683E-426.9293351
GTCTCGC6650.025.795471
GGGTACG901.9265855E-423.3387551
CTGTCGC7150.022.5174149
TCGCTCT6800.022.1348934
GTATAAG3500.022.0051141
TCTCGCT7800.021.541442
CTCGCTC7350.020.9547843
TGTCGCC7300.019.65747510
TCGTAGG900.005274093219.4471322
GTCTTGC12750.018.3964311
GTATATG4150.017.714961
GTATTAG3600.017.5040661
CGCTCTG7700.016.820115
GTGTTAC5500.016.5493011
CGTATCA1503.160791E-416.33328270
GTGTAAG6150.015.93866351
GTCTCAC15600.015.9331891
GTACTAA2653.4891855E-815.8527391
Produced by FastQC (version 0.11.5)