FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483001
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG1253.5652192E-927.99999270
TCTGTCG5850.025.1271868
TGTCGCC6650.022.63066110
GTATAAC2550.021.9665281
CTGTCGC7450.021.1400839
TCGCTCT7400.020.8166754
TACGATA900.0052705819.4495322
GTCTCGC7600.019.3471641
GTACTAT3450.019.2804031
GTCGCCC7100.019.22418411
GTCTTAC6250.018.4848351
GTATAGG3550.017.7511221
GTATAGA3600.017.5045781
TACTGCG1602.6102975E-517.499297
GTATTAG5250.017.3378681
GTCTTGC13950.017.0653951
GCGTAGC1854.7424146E-617.031481
CTCGCTC8850.017.0100983
AATACCG1653.3900542E-516.9744785
TCGCCCA8250.016.5444512
Produced by FastQC (version 0.11.5)