FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069189
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5500.027.9989748
GTACCGT700.001225939125.0033131
TGTCGCC6450.024.9603310
GTCGCCC6550.021.90759511
GTGTTAG8550.021.289371
TCGCCCA6600.021.21185912
CTGTCGC7650.020.5874829
GTATTGA4350.020.1176071
CGCCGTA1751.2668897E-719.99975267
GTATAGA3400.019.5614171
GACGTAA900.00527421919.4470231
CGTATCA1801.704284E-719.44467470
GTCTTGC13200.019.0934411
GTACGGA1107.473219E-419.093441
GTCTCGC5550.018.9214271
GTATTAG3550.018.7348791
GTACCGG1351.4116995E-418.1505551
GTGTTAC4500.017.113381
TCGCTCT5800.016.896754
GTGTAGA12800.016.67988470
Produced by FastQC (version 0.11.5)