FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276729
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6200.024.837368
TGTCGCC6200.023.7083910
GTATAGA3750.021.471161
CTGTCGC7350.021.4274069
GTACTAG2650.021.13651
TCGCCCA7200.019.92947412
GTCGCCC6050.019.66835411
GTCTCGC7200.019.4485151
TCGCTCT7300.019.1812524
GTATAAT3850.019.0949041
ATCTCGT6400.018.59437470
GTCTTGC12050.018.3025861
TATACTG4900.017.8600965
CTCGCTC8200.017.076373
GTATTCG1250.001760843116.8035181
GTATTAA4400.016.7080421
TCTCGCT8000.016.6281152
TAAGACT5900.016.6129174
TTATACT3850.016.3663434
CGCTCTG8000.016.1901785
Produced by FastQC (version 0.11.5)