FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604690
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG1403.289606E-722.50045870
GTATACG952.780167E-422.108261
GTACTAC3100.019.1961481
TCTGTCG6950.019.1363438
TCGCTCT8050.018.6981834
TCCGTAC950.007208155518.4231973
GTCTCGC7850.017.3908921
TTATACT4750.016.9496654
GTATTGG5000.016.8022771
GTATTAA4400.016.7068081
ATCTCGT12700.016.53576970
CACGAGC6450.016.279470
GTCCTAT4700.015.6404171
CTGTCGC9000.015.5552739
GTATAAT4300.015.4672121
TTACACT8400.015.4187564
GTGTATA6850.015.3305441
GTACTAG3452.4556357E-1015.2194531
GTATTAC4450.014.9458451
TAACACG3051.211265E-814.9200544
Produced by FastQC (version 0.11.5)