FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604690
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8250.026.7271488
CTGTCGC10150.023.7929949
GTCGCCC8550.023.33322511
TGTCGCC10650.023.00458710
TCGCCCA9000.022.55544912
GTCTCGC8700.021.7257061
TCGCTCT9750.020.8208144
GTCTTGC17000.020.589721
GTCTTAC7850.019.619251
CGCTCTG9450.019.2595395
GTATTAT3650.019.1794641
GTATAGG3350.018.8073271
CGTATCA2601.4642865E-917.49991870
GTATTAA5050.017.3279821
GTATAAG4650.017.3130761
GCGTAAA1250.001762552316.801211
TCTCGCT10750.016.6052112
GTGTTAA6600.016.4405781
TTGCTCT20150.015.9803814
GTGTTAC5800.015.6907881
Produced by FastQC (version 0.11.5)