FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772147
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7000.024.4988828
GTATAGA4650.023.337321
AATATCG3050.020.65628470
TGTCGCC9050.020.49630210
GCGCTAA900.00527624819.44601239
CTGTCGC9200.019.4012899
GTCGCCC9250.019.29641711
GTCTCGC8700.018.9112781
TCGCTCT8500.018.5325784
GTATATA6750.018.151251
GTATTAC3350.017.7642271
GTATAGG4150.017.713871
TCGCCCA10850.017.4185612
ATCGTTC1653.3998156E-516.96892210
GTCTTGC16100.016.959421
GTATTAT4350.016.8994391
GTATAAG5650.016.7285211
TCTCGCT8900.016.5196762
TTACTCG1300.002289281516.1566074
GTATTAG4800.016.0444091
Produced by FastQC (version 0.11.5)