FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772147
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7450.022.553591
TCTGTCG7350.020.9520038
GGTATAC3300.020.1544251
GTCGTAA1055.463919E-420.0028861
GTCTTGC16650.019.5523721
GTATTAA5850.019.1480621
GTACGGA1107.473504E-419.0936641
TCGCTCT8200.018.7808274
CTGTCGC9500.018.0523059
GTCTTAC6650.017.897321
TAATACT5550.017.6579764
CTCGCTC8600.017.5015773
GTCGCCC7700.017.27241711
CGCCGTA2056.678765E-717.07317268
GTATAAT5800.016.898991
TCTCGCT9150.016.8324242
GTATTAT5250.016.6690731
TGTCGCC9250.016.64834810
GTATATA6600.016.4417671
CGCTCTG8550.015.9651995
Produced by FastQC (version 0.11.5)