FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540241
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG800.002668018521.8750115
GTCTCGC8150.021.476211
TCGCTCT8150.019.7546124
CGCTCTG9200.018.260885
GTCTTGC15650.017.6708721
TAGCGCT1401.8667906E-417.500014
TCTCGCT8450.017.3985142
GTGTATA7650.017.3887121
GTATTAT5300.017.1728651
CTAGTAC2451.3291356E-817.1442173
GTATTAA4650.016.5620841
CTCGCTC9100.016.5397723
GTGTTAG11250.016.1806551
GGCGTAT1300.002289105416.1567171
TACACTG10050.016.019915
GTATTAG5050.015.9434271
GTATAGC3351.5643309E-1015.6744271
GTATTAC4250.015.6498391
GTACAAG6300.015.5583221
TCTGTCG7450.015.5027548
Produced by FastQC (version 0.11.5)