FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202461
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6050.025.4542888
TGTCGCC6500.024.23052610
CTGTCGC6750.023.851619
GTCGCCC6550.021.90817611
GTATAGG3700.021.7595021
TCGCTCT6150.021.0571774
GTCCGTA1003.931859E-421.002651
GTACGGT1003.931859E-421.002651
GTCTCGC5550.020.8134371
GTCTTGC12250.020.2882731
GTACCGT900.00527867619.444256
TTATACT3900.018.8463924
GCCGACC4650.018.81744270
CCGACTA950.007213067718.42086860
GTATTAC3250.018.3100011
CGCCGTA1150.001008331518.261168
TCGCCCA7900.017.7209412
GGATACG1000.009686070517.5022091
GTATTAG4600.017.5022071
TACGACA1000.0096936717.49982529
Produced by FastQC (version 0.11.5)