FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225380
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6800.026.7702061
TCTGTCG7250.023.1704068
GTATAGA3600.022.3657051
TCGCTCT8050.022.1774734
TAATACT3450.021.3077664
GTCTTGC13500.021.2636281
CTCGCTC8800.021.0834033
TGTCGCC8450.020.70826310
GTCGCCC7100.020.70289411
GTATAGG3250.020.4657421
TCTCGCT8900.020.0598492
CTGTCGC9000.019.8316149
GTATTGG5000.019.6040271
GTATAAG3950.019.4976771
GTATTAG4900.019.2896771
CGCTCTG8900.019.2727575
GCCGAAC950.00720412918.4248371
GTGTAAA7450.018.3259141
GTGTATA6150.018.2151241
GTATTAA3900.017.9524061
Produced by FastQC (version 0.11.5)