FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411219
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4750.022.842418
ATCTCGT7000.022.49983470
TAATACG1102.9197286E-522.2781074
GTCTCGC6250.019.6047341
TATCTCG8050.019.56507169
TAATCGG900.00528000419.44349522
GTGTTAG9650.019.227441
GTATTGG5200.018.1774671
GTAATAA4350.017.7054251
GTGTTAA5150.017.6741711
GTATTAT4600.017.5042271
CTACGCT1000.009679900517.5042274
GTCTTGC14450.017.2013851
GTATTAC3700.017.031141
GGTATAC2702.408342E-916.8559231
GTATAAG5100.016.4745661
TCGCTCT7250.016.4177574
GTATAAC3005.529728E-1016.3372781
CGTTTCG1554.057677E-415.8063359
TCTCGTA4450.015.73022170
Produced by FastQC (version 0.11.5)