FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181470
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6350.027.557528
CTGTCGC8000.023.6242289
GTACGTA750.001830972423.338991
TGTCGCC7650.022.87506910
GTCTTAC4850.020.9329071
GTCTTGC13400.020.639331
GTACACG1601.1846569E-619.6922721
CGTTAGA1257.815902E-519.6047522
GTATAAT2504.1836756E-1119.6047521
TCGCCCA7200.019.44336112
ATTAGAC3650.018.2235953
GTACTAA2551.1259544E-917.8474621
GTATTAG3950.017.7258151
GTCTAGG6350.017.642071
GTGTAAA7750.017.6171721
GTCTCGC7950.017.6143321
GTATAGC2601.4588295E-917.5042421
AATATCG1401.8661324E-417.50063170
GTCTCAC16200.017.288141
GTGTATA5150.016.994411
Produced by FastQC (version 0.11.5)