FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478920
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14200.042.5839738
GTCTCGC12750.034.80561
GTCTTGC27300.033.3101731
CTGTCGC22850.027.723739
TCGCTCT14000.027.2522934
GTGTAAC8850.025.4828471
TAATACT8950.024.9341094
TTCACCG8700.024.8231685
GTCTCAC34400.024.5317821
TACACTG21500.024.4421625
TCTCGCT15350.024.3865382
TTGCTCT37200.024.3827574
GTATTAG10450.023.6696641
CTCGCTC16800.023.5672343
ACCGTGT11250.023.03598
GTGTTAC12650.023.0037671
GGTATAC7350.022.7651211
GTGTAAA18400.022.5365051
GTCTCGA27950.022.4074616
GTATTAT9750.022.3844381
Produced by FastQC (version 0.11.5)