FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478920
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14550.038.45761
TCTGTCG13500.036.2610668
GTCTTGC26650.034.357991
TCGCTCT15200.033.6259044
CTCGCTC17500.029.631063
TCTCGCT18650.028.976192
TACGACT1804.9710907E-627.9952744
GTCTTAC12650.026.9998741
CGCTCTG19050.026.4522295
TACCCCG3052.197703E-823.6025735
GTACTAC5900.023.4021641
TCTGTCA38850.023.3477448
GAGTGCA80750.023.1787516
GGAGTGC74100.023.0244525
GTGTTAG18000.023.0121291
GTATTAC7300.022.8959871
CTTGCTC35500.022.7217833
TAACACG6350.022.6733384
GTTAGCC14950.022.641723
CTGGAGT86350.022.5192623
Produced by FastQC (version 0.11.5)