FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239452
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.039.2109538
TCGCTCT14000.035.477974
GTCTTGC25200.034.347781
GTCTCGC12450.033.3010371
TAATACT8350.030.1730544
GTATAAC5100.029.9503141
GTACTAT6850.029.7316991
TCTCGCT14100.029.6105582
GTATTAC7250.029.0945911
GTCTTAC10150.027.9480061
CTCGCTC15000.027.8339233
CGCTCTG16100.027.273555
GTGTTAC10650.025.952921
GTGCTAC8750.025.7694911
TAGTACT6750.024.5279794
GTCTCAC31250.024.20671
CTACGCT2701.7691491E-723.9947624
CTTGCTC31950.023.20623
GGAGTGC66950.023.1166865
GAGTGCA70150.023.088336
Produced by FastQC (version 0.11.5)