FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4209067
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15800.040.099198
GTCTTGC31700.036.1318661
GTCTCGC16800.032.5879361
CTGTCGC21950.031.4881159
TCGCTCT16600.029.4924474
GTGTTAC14850.028.1354641
TCTCGCT20150.025.011412
TACACTG23050.024.987865
GTATTAA11950.024.7154481
TTACACT18800.024.126414
TTGCTCT43350.023.9157164
CGCTCTG20150.023.5819075
GTGCTAC13450.023.565841
CTCGCTC20900.023.4246223
GTGTTAT15750.023.3261031
GGTATAC8350.023.2932361
CTTGCTC43850.023.1504543
GTATTGC10350.022.9681551
GGAGTGC85950.022.951695
CTCGAAC25900.022.7941868
Produced by FastQC (version 0.11.5)