FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921921
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10100.034.9295778
GTCTTGC22800.032.2293551
GTCTTAC9850.028.5242751
CTGTCGC13450.027.8355439
TCGCTCT10500.027.4276944
GTCTCGC9850.027.0614911
GCGTAGT3409.822543E-1125.4266171
TTGCTCT29700.025.2113174
GTGTAAG11850.024.9259531
GTTACCC8700.024.8267923
TACCCGT2951.5399564E-824.4065
CTGTTGC26450.024.226079
CGTTTCG2701.7658385E-723.9992319
TCTGTTG26350.023.2250638
GTATAGG7250.022.854731
GTGTTAG14850.022.8011971
GTACTGA9500.022.7501321
GTGTAGG16150.022.750131
GTATTAA7300.022.698191
CTTGCTC28750.022.538413
Produced by FastQC (version 0.11.5)