FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569366
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15050.042.1199871
TCTGTCG13500.035.732558
TCGCTCT16050.034.5412984
CTCGCTC16600.033.3968623
TCTCGCT19400.032.6590772
CGCTCTG17600.030.2720645
GTCTTAC12250.029.9899771
CTGTCGC19150.028.9497579
GTCTTGC29300.028.5188751
GTGTTAG22600.028.3676681
GTTCTAC9050.025.470851
GTATTAG11150.024.5499611
GGCTAGA18550.023.6879771
CGGTACT2151.9705221E-523.4413474
GTCTCAC35650.023.2370171
GTTAGCC18400.023.0864523
GGTATAC6250.023.051121
GAGTGCA76700.022.9043246
GGATAGG14250.022.7478161
GTATTAT11150.022.6118071
Produced by FastQC (version 0.11.5)