FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678419
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.047.621258
GTCTCGC13650.039.0323141
TCGCTCT14850.036.3630264
CTGTCGC18050.035.5007939
TCTCGCT16000.031.4994752
GTCTTGC31200.031.1533241
GTATAAT9950.030.391451
CTCGCTC16700.029.7480053
CGCTCTG19200.028.1245295
GTATTAT11450.025.7812291
GTCTTAC12400.025.547961
GTATTAC8200.025.4629861
GGCTAGA21750.025.1582011
TTGCTCT41100.024.8754964
GGGTTTC53300.023.9095441
GTGTAGG18500.023.7401451
GTGTTAC13650.023.7358681
GTGTAAG16500.023.563241
TCCGTCG1851.920145E-423.3509628
GTATAAG10950.023.0133151
Produced by FastQC (version 0.11.5)