FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623769
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13300.043.3087238
GTCTCGC13650.039.5603071
TCGCTCT15250.035.8823434
GTCTTGC28750.033.8085821
CTGTCGC18200.030.4614379
TCTCGCT17000.029.2238352
CTCGCTC18650.028.18263
GTGTTAG23550.028.1272951
CGCTCTG18900.026.6669455
GTATTAG10900.026.421931
TTGCTCT42550.025.043744
TCTGTCA39600.024.545718
TCGATCT14900.024.1609949
CTTGCTC37750.024.032043
GGCTAGA20400.023.9999181
GTGTAAA19750.023.6961231
GTCTCAC38600.023.6890411
TTACACT15200.023.6844584
GGAGTGC75400.023.5864545
GGCGTAC2151.9702495E-523.4417841
Produced by FastQC (version 0.11.5)