FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459481
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC28300.036.9185521
GTCTCGC13650.035.367571
TCTGTCG14250.033.851488
CTGTCGC18850.031.3198879
TCGCTCT15300.030.5871964
GTATTAG10350.026.4549121
GTATAAG10100.026.3963241
TCTCGCT17550.025.0255152
GTCTCAC34350.024.9622271
GTATAGG8450.024.7288761
CGCTCTG17250.024.2078725
CTCGCTC17150.023.9292143
TTGCTCT37750.023.8402484
GTATAGC7050.023.5072481
GTGTTAG20550.023.4923271
GAGTGCA74700.023.420896
GTATTAC7700.023.3944241
GGCTAGA16950.022.955521
GGAGTGC69850.022.8825445
GTCTTAC13300.022.7541561
Produced by FastQC (version 0.11.5)