FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468183
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.036.1741338
GTCTCGC13900.032.632821
TCGCTCT15050.030.1397154
GTATTAC8950.027.3517251
GTCTTGC28100.026.3912371
TCTCGCT15950.026.1819742
GTACTAG6600.026.1815991
CGCTCTG16700.025.437285
CTGTCGC15350.024.3906759
GTGTAGA16900.024.2838171
GGTATAC7550.023.8408581
GTATTAT12200.023.606361
GTGCGAA3102.6069756E-823.225611
TTCACCG10100.022.812025
GTATAAT10500.022.628381
GTGTTAG23450.022.4134581
GTATTAG12850.022.4122621
CTCGCTC17700.022.3730163
GGGTTTC48850.022.25571
ACCGTGT11850.021.8735498
Produced by FastQC (version 0.11.5)