FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468183
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12100.038.0815968
GTCTCGC12800.037.710931
TCGCTCT14550.032.1640624
GTCTTGC30250.029.7705611
TCTCGCT16000.029.2491912
CTGTCGC17300.028.7159739
CGCTCTG16450.027.573715
CTCGCTC16200.027.1103653
GGTATAC7000.026.7594811
GTGTAGG16350.024.235241
GTCTCGA26900.023.8209066
GTGTTAC13300.023.2926671
GTGTTAG21550.022.7333811
GTATTAG10550.022.5353261
GTGTAAG15100.022.4245261
GGTCTCG29300.022.3611585
TCGAACT19000.022.3572799
TTGCTCT39450.022.0830424
TATACTG9850.021.928335
GTATAAC6900.021.9266641
Produced by FastQC (version 0.11.5)