FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379769
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.043.6534088
GTCTCGC12300.038.6334571
CTGTCGC15700.035.3114749
GTCTTGC27900.033.2897261
TCGCTCT13400.032.7760164
TCTCGCT15800.029.1644692
TTGCTCT37150.026.9393524
GTATTAC7950.026.2636811
CGCTCTG16500.025.7453775
GTCTTAC12100.025.5863191
CTCGCTC16500.025.3090133
GTGTTAG20400.025.0583741
GTGTAAC9300.024.773751
CTTGCTC37150.023.8384193
TATACTG11500.023.791235
GTCTCAC33300.023.3509351
GGGTTAC9500.022.7364351
TACACTG19750.022.2379075
CTATACT8500.022.0234624
GTGTAAT13750.021.9923341
Produced by FastQC (version 0.11.5)