FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3368565
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13200.044.7276461
TCTGTCG10900.040.2933168
GTCTTGC25050.036.50331
TCGCTCT15100.035.7613564
TCTCGCT16150.033.8821332
CTCGCTC16300.033.128623
GTCTTAC11550.031.1690921
CTGTCGC17400.030.20679
GTATTAC7450.029.959981
GTGTTAG19050.027.2128261
TTACACT14700.026.93864
CGCTCTG17150.026.4488095
TTGCTCT37250.026.0937884
TCTGTCA36750.025.4692218
GTCTCAC32850.025.4248681
CTTGCTC34900.025.3751933
GGGTTTC43300.024.942471
GTATTAA8700.024.8277931
GTATTAT7750.024.155041
GTACAGG15050.023.9204651
Produced by FastQC (version 0.11.5)