FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687168
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15650.043.2462841
TCTGTCG13000.042.645858
TCGCTCT16650.038.0537874
CGCTCTG18000.034.3997575
TCTCGCT18600.034.0642742
GTCTTGC27850.033.0917741
CTCGCTC19400.032.2884333
CTGTCGC17900.031.374089
GTATTAG12550.027.5380291
GTATTAT11600.026.689831
GTGTAAA22150.025.3545671
GGTTAAC6900.025.0436421
GTCTTAC11600.024.827751
GGGTTTC48200.024.796841
TTGCTCT38050.024.7882614
GTGTACG4650.024.7743551
GTACTGA11700.024.6155451
GTACTAC6150.024.5855271
GTCTCAC35600.024.4720691
GTTTCAC45600.023.999833
Produced by FastQC (version 0.11.5)