FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347449
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.041.3184938
TCGCTCT13400.037.0717124
GTCTTGC26800.037.0717121
GTCTCGC12250.035.2625351
CTGTCGC14750.032.2144139
TTGCTCT35350.027.9016274
CGCTCTG16400.027.656365
TCTCGCT14900.027.0582732
CTCGCTC15600.026.7671263
TAATACT11250.026.2379364
GTATTAC7300.025.641821
GTCTTAC11050.024.7582341
TCTGTCA33800.023.8561138
GTATAGC8500.023.7157841
CTTGCTC38150.023.5892333
GTGCTAC11650.023.483131
GTCTCAC32500.023.481231
GTGTTAC12550.022.9464021
TCTTGCT42700.022.9302832
GTATTGG10450.022.7350541
Produced by FastQC (version 0.11.5)