FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3347449
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.045.0771988
GTCTTGC26850.033.7879261
CTGTCGC18050.029.9161939
GTCTCGC13350.028.584451
GTCTTAC12400.026.7098461
TCGCTCT13500.026.6668344
GTCTCAC32700.025.9818151
GTGTTAG20750.025.330281
GTCTTAA10750.024.7815511
GGTATAC6400.024.7501561
TTACTCT15800.024.607754
GCGTTAC2051.3625189E-524.5855221
TACACTG17700.024.4069355
GTATAGC7400.024.324481
GTATAGG8900.024.2698151
GTGTTAC12800.024.1876531
TAGTACT7400.023.35154
GTGTAAG13950.023.2259541
TCTCGCT15850.023.167342
CGCTCTG16150.022.7369865
Produced by FastQC (version 0.11.5)