FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010413
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.039.1291128
TCGCTCT11700.038.767924
GTCTCGC11800.033.558191
GTCTTGC24100.032.861961
TCTCGCT14000.031.3703672
CGCTCTG13650.031.1198255
CTCGCTC13850.029.630773
CTGTCGC12050.029.277029
GTCTTAC12150.027.2583391
GTGTTAC11800.026.846551
GTGTTAG18550.025.6163851
GTACTAG5100.025.4109061
GTATTAT10000.023.7591971
GTATTAG10650.022.9851381
GTACGGG2552.8787854E-622.5874711
GTACCGG3203.6507117E-822.4992391
TTACTCT15550.022.224334
CTGTTGC26300.021.90049
GTCTCAC30500.021.4812411
CGGTACT2353.9169034E-521.4460834
Produced by FastQC (version 0.11.5)