FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816969
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10100.043.484668
GTCTCGC11550.041.7665061
TCGCTCT12000.040.799544
GTCTTGC22650.038.1459471
CTCGCTC13450.035.330463
CTGTCGC14100.034.7230159
GTCTTAC9600.033.0002141
TCTCGCT14300.032.7269062
CGCTCTG13000.029.9073565
GGGTTTC38650.028.5021271
GTATAAG8100.027.5557371
ACCGTGT8050.026.8319978
TAACCCG2156.5138556E-726.79045
GTGTTAT9000.026.4001711
GTATTAA8050.025.9380571
TTCACCG7850.025.6812425
CGTTTCG1701.0817115E-425.4114789
TTGCTCT29300.025.3102974
GTTACAC8850.025.2200573
GTCCTAT6600.025.0910741
Produced by FastQC (version 0.11.5)