FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090564
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.050.7842038
GTCTCGC13400.039.760011
CTGTCGC15250.037.7693679
TCGCTCT14100.037.2754864
GTCTTGC27100.035.0690651
CTCGCTC14600.033.0401153
TCTCGCT14950.032.2665982
CGCTCTG14700.031.3460045
TTGCTCT33300.027.674854
GTCTCAC28100.027.6717761
GTCTAGA11800.026.8466591
GTGTAAC9400.026.8077131
GTGTTAC11050.026.7141361
TCTGTCA34300.024.978858
CTTGCTC33400.024.7896823
TCTCGAT12100.024.3959677
CTCGATC11850.024.3030768
GTATAAT9700.023.751871
TCGATCT12200.023.6058569
GGGTTTC44000.023.5629351
Produced by FastQC (version 0.11.5)