FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077077
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.037.2611858
GTCTTGC25950.036.0967831
GTCTCGC12800.032.0868721
CTGTCGC14050.030.7457039
GTATAAG9200.029.7617381
GTCTTAC12800.028.146381
TCGCTCT12550.028.1109814
GTGTTAG22100.027.7133581
GTATTAC7400.027.2639521
GTATAAA12150.026.686941
GTCTCAC31900.026.6534731
TCTCGCT14250.025.788012
GTATTAG10050.024.3767241
TTGCTCT36900.023.8043944
GTATAAC6100.023.6245021
CTTGCTC36850.022.6820873
ACCGTGT9850.022.6586998
GTTTCAC42900.022.3386463
GGGTTTC47500.022.147351
GTACTAT7550.021.950451
Produced by FastQC (version 0.11.5)